Journal: Journal of Neuroinflammation

Article Title: Immunoglobulin G (IgG) attenuates neuroinflammation and improves neurobehavioral recovery after cervical spinal cord injury

doi: 10.1186/1742-2094-9-224

Figure Lengend Snippet: List of primary and secondary antibodies used for western blot analysis

Article Snippet: Anti-rat matrix metalloproteinase (MMP)-9 , Rabbit , Chemicon (AB19016) , 1:1,000 , Overnight 4°C.

Techniques: Western Blot

Journal: Journal of Neuroinflammation

Article Title: Immunoglobulin G (IgG) attenuates neuroinflammation and improves neurobehavioral recovery after cervical spinal cord injury

doi: 10.1186/1742-2094-9-224

Figure Lengend Snippet: IgG reduces MMP-9 expression. ( A ) Matrix metalloproteinase-9 (MMP-9) expression was determined by western blot analysis at 24 h after SCI. Three treatment groups were studied including laminectomy only (sham, n = 4), intravenous saline (saline, n = 9), and intravenous IgG (IgG, n = 8). IgG treatment was associated with a significant reduction in MMP-9 level in the injured spinal cord ( P = 0.004). Error bars represent SEM. ( B ) A representative western-blot of MMP-9 is shown. β-actin was used as a loading control.

Article Snippet: Anti-rat matrix metalloproteinase (MMP)-9 , Rabbit , Chemicon (AB19016) , 1:1,000 , Overnight 4°C.

Techniques: Expressing, Western Blot

Journal: Diagnostic Pathology

Article Title: Dramatic early event in chronic allograft nephropathy: increased but not decreased expression of MMP-9 gene

doi: 10.1186/1746-1596-8-13

Figure Lengend Snippet: MMP mRNA expression in allografts and controls. Analyzed by densitometric comparison to β-actin (internal control) from the same sample after the positive image was digitized by video for computerized densitometry. The results were given as a ratio of the intensity of MMP-9 mRNA and β-actin mRNA. The expression MMP-9 mRNA was significantly increased in allografs compared to either F344 controls (P < 0.05) or LEW controls (P < 0.05).

Article Snippet: Goat anti-rat polyclonal antibodies MMP-9 and TGF-beta1 are purchased from Santa Cruz (U.S.A.) and for secondary and tertiary staining from DAKO (Denmark).

Techniques: Expressing

Journal: Diagnostic Pathology

Article Title: Dramatic early event in chronic allograft nephropathy: increased but not decreased expression of MMP-9 gene

doi: 10.1186/1746-1596-8-13

Figure Lengend Snippet: Immunohistochemical localization of MMP-9 and TGF-beta1 in the kidney of allografts and controls. Immunohistochemical analysis showed that MMP-9 and TGF-beta1 stained in tubulointerstitium and arteriolar walls of allografts and controls ( A and C are MMP-9 staining in allografts, B and D are controls. E and G are TGF-beta1 staining in allografts, F and H are controls).

Article Snippet: Goat anti-rat polyclonal antibodies MMP-9 and TGF-beta1 are purchased from Santa Cruz (U.S.A.) and for secondary and tertiary staining from DAKO (Denmark).

Techniques: Immunohistochemical staining, Staining

Journal: Diagnostic Pathology

Article Title: Dramatic early event in chronic allograft nephropathy: increased but not decreased expression of MMP-9 gene

doi: 10.1186/1746-1596-8-13

Figure Lengend Snippet: The expression of MMP-9 and TGF-beta1 in allografts and controls. A Expression of MMP-9 in the tubulointerstium of allografts and controls (100 ×). . The immunostaining of MMP-9 in tubulointerstitium of allografts (0.325 ± 0.093) significantly increased compared to either F344 controls (0.174 ± 0.052) and LEW controls (0.121 ± 0.047). B Expression of MMP-9 in the arteriolar wall of allografts and controls (100 ×). The immunostaining of MMP-9 in arteriolar wall of allografts significantly increased compared to F344 control. C Expression of TGF-beta1 in the tubulointerstium of allografts and controls (100 ×).The immunostaining of TGF-beta1 in tubulointerstitium of allografts significantly increased compared to either F344 controls or LEW controls. D Expression of TGF-beta1 in the arteriolar wall of allografts and controls (100 ×). The immunostaining of TGF-beta1 in arteriolar wall of allografts significantly increased compared to either F344 controls or LEW controls.

Article Snippet: Goat anti-rat polyclonal antibodies MMP-9 and TGF-beta1 are purchased from Santa Cruz (U.S.A.) and for secondary and tertiary staining from DAKO (Denmark).

Techniques: Expressing, Immunostaining

Journal: Diagnostic Pathology

Article Title: Dramatic early event in chronic allograft nephropathy: increased but not decreased expression of MMP-9 gene

doi: 10.1186/1746-1596-8-13

Figure Lengend Snippet: The correlation between the expression of MMP-9 mRNA and degree of mononuclear cells infiltration in allografts. MMP-9 mRNA level of allografts was demonstrated by MMP-9 mRNA/β-actin mRNA ratio, the degree of interstitial mononuclear cell infiltration was evaluated according to Banff Criteria, as follow, 0 = No or trivial interstitial inflammation; 1 = up to 25% of parenchyma inflamed; 2 = 26% to 50% parenchyma inflamed; 3 = > 50% parenchyma inflamed. The result of bivariate non-parameter correlation analysis demonstrated: the MMP-9 mRNA levels of allografts were correlated to the degrees of interstitial mononuclear cell infiltration. (P < 0.05, Spearman correlation coefficient was 0.653).

Article Snippet: Goat anti-rat polyclonal antibodies MMP-9 and TGF-beta1 are purchased from Santa Cruz (U.S.A.) and for secondary and tertiary staining from DAKO (Denmark).

Techniques: Expressing

Journal: Diagnostic Pathology

Article Title: Dramatic early event in chronic allograft nephropathy: increased but not decreased expression of MMP-9 gene

doi: 10.1186/1746-1596-8-13

Figure Lengend Snippet: The potential role of MMP in earlier stage of CAN. A The correlation between the immunostaining of MMP-9 in tubulointerstitium and the quantity of interstitial mononuclear cells in allografts. The immunostaining of MMP-9 in tubulointerstitium was correlated to the quantity of interstitial mononuclear cells in allografts (P < 0.05, Spearman correlation coefficient was 0.757). B The correlation between the immunostaining of MMP-9 in arteriolar wall and the quantity of vascular SMCs in allografts. The immunostaining of MMP-9 in arteriolar wall was correlated to the quantity of vascular SMC in allografts (P < 0.05, Spearman correlation coefficient was 0.741). C The correlation between the immunostaining of MMP-9 and TGF-beta1 in the tubulointerstitium of allografts. The immunostaining of MMP-9 and TGF-beta1in tubulointerstitium in allografts have correlation. (P < 0.01, Spearman correlation coefficient was 0.810). D The correlation between the immunostaining of MMP-9 and TGF-beta1 in arteriolar wall of allografts. The immunostaining of MMP-9 and TGF-beta1 in arteriolar wall of allografts have correlation. (P < 0.001, Spearman correlation coefficient was 0.958).

Article Snippet: Goat anti-rat polyclonal antibodies MMP-9 and TGF-beta1 are purchased from Santa Cruz (U.S.A.) and for secondary and tertiary staining from DAKO (Denmark).

Techniques: Immunostaining